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Proceedings Paper

Determination of calcium concentrations in cells and tissue with fluorescence lifetime imaging (FILM)
Author(s): T. Gensch; M. Wirth
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Paper Abstract

The determination of ion concentrations in cells - in particular in neurons - is very important for understanding cell function and life. Calcium is an ubiquitous messenger in almost all cell types. Fluorescence lifetime imaging (FLIM) can be of advantage over intensity based fluorescence microscopy, when comparisons between micro-domains of one cell or between different cells of one cell type are performed. Several organic chromophores have been tested in cuvette experiments as well as in living cells and cell tissue with respect to their applicability in FLIM studies. The calcium concentration changes in several cell types were investigated by FLIM with two-photon excitation.

Paper Details

Date Published: 28 February 2011
PDF: 15 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 790322 (28 February 2011); doi: 10.1117/12.874507
Show Author Affiliations
T. Gensch, Forschungszentrum Jülich GmbH (Germany)
M. Wirth, RWTH Aachen (Germany)


Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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