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Proceedings Paper

In vivo experimental study on laser welded ICG-loaded chitosan patches for vessel repair
Author(s): Francesca Rossi; Paolo Matteini; Giuseppe Esposito; Alessio Albanese; Alfredo Puca; Giulio Maira; Giacomo Rossi; Roberto Pini
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Paper Abstract

Laser welding of microvessels provides several advantages over conventional suturing techniques: surgical times reduction, vascular healing process improvement, tissue damage reduction. We present the first application of biopolymeric patches in an in vivo laser assisted procedure for vessel repair. The study was performed in 20 New Zealand rabbits. After anesthesia, a 3-cm segment of the right common carotid artery was exposed and clamped proximally and distally. A linear lesion 3 mm in length was carried out. We used a diode laser emitting at 810 nm and equipped with a 300 μm diameter optical fiber. To close the cut, ICG-loaded chitosan films were prepared: chitosan is characterized by biodegradability, biocompatibility, antimicrobial, haemostatic and wound healing-promoting activity. ICG is an organic chromophore commonly used in the laser welding procedures to mediate the photothermal conversion at the basis of the welding effect. The membranes were used to wrap the whole length of the cut, and then they were welded in the correct position by delivering single laser spots to induce local patch/tissue adhesion. The result is an immediate closure of the wound, with no bleeding at clamps release. The animals were observed during follow-up and sacrificed after 2, 7, 30 and 90 days. All the repaired vessels were patent, no bleeding signs were documented. The carotid samples underwent histological examinations. The advantages of the proposed technique are: simplification of the surgical procedure and shortening of the operative time; good strength of the vessel repair; decreased foreign-body reaction, reduced inflammatory response and improved vascular healing process.

Paper Details

Date Published: 18 February 2011
PDF: 5 pages
Proc. SPIE 7883, Photonic Therapeutics and Diagnostics VII, 78833M (18 February 2011); doi: 10.1117/12.874460
Show Author Affiliations
Francesca Rossi, Institute of Applied Physics, Nello Carrara, CNR (Italy)
Paolo Matteini, Institute of Applied Physics, Nello Carrara, CNR (Italy)
Giuseppe Esposito, Catholic Univ. (Italy)
Alessio Albanese, Catholic Univ. (Italy)
Alfredo Puca, Catholic Univ. (Italy)
Giulio Maira, Catholic Univ. (Italy)
Giacomo Rossi, Univ. of Camerino (Italy)
Roberto Pini, Istituto di Fisica Applicata Nello Carrara (Italy)

Published in SPIE Proceedings Vol. 7883:
Photonic Therapeutics and Diagnostics VII
Kenton W. Gregory; Nikiforos Kollias; Andreas Mandelis; Henry Hirschberg; Hyun Wook Kang; Anita Mahadevan-Jansen; Brian Jet-Fei Wong; Justus F. R. Ilgner; Bodo E. Knudsen; E. Duco Jansen; Steen J. Madsen; Guillermo J. Tearney; Bernard Choi; Haishan Zeng; Laura Marcu, Editor(s)

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