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Proceedings Paper

Classification of bacterial samples as negative or positive for a UTI and antibiogram using surface enhanced Raman spectroscopy
Author(s): Evdokia Kastanos; Katerina Hadjigeorgiou; Alexandros Kyriakides; Costas Pitris
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Paper Abstract

Urinary tract infection (UTI) diagnosis requires an overnight culture to identify a sample as positive or negative for a UTI. Additional cultures are required to identify the pathogen responsible for the infection and to test its sensitivity to antibiotics. A rise in ineffective treatments, chronic infections, rising health care costs and antibiotic resistance are some of the consequences of this prolonged waiting period of UTI diagnosis. In this work, Surface Enhanced Raman Spectroscopy (SERS) is used for classifying bacterial samples as positive or negative for UTI. SERS spectra of serial dilutions of E.coli bacteria, isolated from a urine culture, were classified as positive (105-108 cells/ml) or negative (103-104 cells/ml) for UTI after mixing samples with gold nanoparticles. A leave-one-out cross validation was performed using the first two principal components resulting in the correct classification of 82% of all samples. Sensitivity of classification was 88% and specificity was 67%. Antibiotic sensitivity testing was also done using SERS spectra of various species of gram negative bacteria collected 4 hours after exposure to antibiotics. Spectral analysis revealed clear separation between the spectra of samples exposed to ciprofloxacin (sensitive) and amoxicillin (resistant). This study can become the basis for identifying urine samples as positive or negative for a UTI and determining their antibiogram without requiring an overnight culture.

Paper Details

Date Published: 11 February 2011
PDF: 6 pages
Proc. SPIE 7911, Plasmonics in Biology and Medicine VIII, 791107 (11 February 2011); doi: 10.1117/12.874425
Show Author Affiliations
Evdokia Kastanos, Univ. of Nicosia (Cyprus)
Katerina Hadjigeorgiou, Univ. of Cyprus (Cyprus)
Alexandros Kyriakides, Univ. of Cyprus (Cyprus)
Costas Pitris, Univ. of Cyprus (Cyprus)

Published in SPIE Proceedings Vol. 7911:
Plasmonics in Biology and Medicine VIII
Tuan Vo-Dinh; Joseph R. Lakowicz, Editor(s)

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