Share Email Print
cover

Proceedings Paper

Single-cell imaging detection of nanobarcoded nanoparticle biodistributions in tissues for nanomedicine
Author(s): Trisha Eustaquio; Christy L. Cooper; James F. Leary
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

In nanomedicine, biodistribution studies are critical to evaluate the safety and efficacy of nanoparticles. Currently, extensive biodistribution studies are hampered by the limitations of bulk tissue and single-cell imaging techniques. To ameliorate these limitations, we have developed a novel method for single nanoparticle detection that incorporates a conjugated oligonucleotide as a "nanobarcode" for detection via in situ PCR. This strategy magnifies the detection signal from single nanoparticles, facilitating rapid evaluation of nanoparticle uptake by cell type over larger areas. The nanobarcoding method can enable precise analysis of nanoparticle biodistributions and expedite translation of these nanoparticles to the clinic.

Paper Details

Date Published: 12 February 2011
PDF: 11 pages
Proc. SPIE 7910, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III, 79100O (12 February 2011); doi: 10.1117/12.874290
Show Author Affiliations
Trisha Eustaquio, Purdue Univ. (United States)
Christy L. Cooper, Purdue Univ. (United States)
James F. Leary, Purdue Univ. (United States)


Published in SPIE Proceedings Vol. 7910:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

© SPIE. Terms of Use
Back to Top