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Proceedings Paper

An integrated microfluidic biosensor for the rapid screening of foodborne pathogens by surface plasmon resonance imaging
Author(s): Michael D. Zordan; Meggie M. G. Grafton; James F. Leary
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Paper Abstract

The rapid detection of foodborne pathogens is of vital importance to keep the food supply rid of contamination. Previously we have demonstrated the design of a hybrid optical device that performs real-time surface plasmon resonance (SPR) and epi-fluorescence imaging. Additionally we have developed a biosensor array chip that is able to specifically detect the presence of two known pathogens. This biosensor detects the presence of the pathogen strains by the selective capture of whole pathogens by peptide ligands functionalized to the spots of the array. We have incorporated this biosensor array into a self contained PDMS microfluidic chip. The enclosure of the biosensor array by a PDMS microfluidic chip allows for a sample to be screened for many strains of pathogens simultaneously in a safe one time use biochip. This disposable optical biochip is inserted into with the hybrid SPR/epi-fluorescence imaging device to form an integrated system for the detection of foodborne pathogens. Using this integrated system, we can selectively detect the presence of E. coli 0157:H7 or S. enterica in a simultaneously in real-time. Additionally, we have modeled the mechanical properties of the microfluidic biochip in order to manipulate the flow conditions to achieve optimal pathogen capture by the biosensor array. We have developed an integrated system that is able to screen a sample for multiple foodborne pathogens simultaneously in a safe, rapid and label-free manner.

Paper Details

Date Published: 8 February 2011
PDF: 10 pages
Proc. SPIE 7888, Frontiers in Biological Detection: From Nanosensors to Systems III, 78880R (8 February 2011); doi: 10.1117/12.874277
Show Author Affiliations
Michael D. Zordan, Purdue Univ. (United States)
Meggie M. G. Grafton, Purdue Univ. (United States)
James F. Leary, Purdue Univ. (United States)


Published in SPIE Proceedings Vol. 7888:
Frontiers in Biological Detection: From Nanosensors to Systems III
Benjamin L. Miller; Philippe M. Fauchet, Editor(s)

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