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Proceedings Paper

Fiber-based combined optical coherence and multiphoton microscopy
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Paper Abstract

This manuscript demonstrates a multimodal imaging system which combined multiphoton microscopy (MPM) imaging modality with Fourier domain (FD) optical coherence microscopy (OCM) imaging modality. The system used a single fiber-based femtosecond laser as the light source for both MPM and OCM modality. The femtosecond fiber laser has a central wavlength of 1.03 μm, a pulse width of 120 fs and a bandwidth of 29 nm. The systems used fiber-based devices for both MPM and OCM imaging. The MPM and OCM shared the same excitation light path. The excitation light was delivered with the core of a dual-clad fiber. The MPM and OCM signal was collected by different parts of the dual-clad fiber. The MPM signal was collected by the clad of the dual-clad fiber and the OCM signal was collected by the core of the dual-clad fiber. The FD OCM used a home-built InGaAs detector array spectrometer with a maximum Aline speed of 7.7 KHz. The multiphoton signal collection efficiency was analyzed and several imaging modality including second harmonic generation imaging, two-photon excited fluorescence and optical coherent microscopy imaging were demonstrated.

Paper Details

Date Published: 10 February 2011
PDF: 7 pages
Proc. SPIE 7892, Multimodal Biomedical Imaging VI, 78920A (10 February 2011); doi: 10.1117/12.874267
Show Author Affiliations
Gangjun Liu, Univ. of California, Irvine (United States)
Zhongping Chen, Univ. of California, Irvine (United States)

Published in SPIE Proceedings Vol. 7892:
Multimodal Biomedical Imaging VI
Fred S. Azar; Xavier Intes, Editor(s)

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