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Proceedings Paper

Imaging site-specific peptide-targeting in tumor tissues using spectral-domain optical coherence tomography
Author(s): Lixin Ma; Miao Zhang; Ping Yu
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Paper Abstract

We report imaging studies on site-specific peptide-targeting in tumor tissues using newly developed optical peptide probes and spectral-domain optical coherence tomography (SD-OCT). The system used two broadband superluminescent light emission diodes with different central wavelengths. An electro-optic modulation in the reference beam was used to get full-range deep imaging inside tumor tissues. The optical probes were based on Bombesin (BBN) that is a fourteen amino acid peptide. BBN has high binding affinity to gastrin-releasing peptide (GRP) receptors overexpressed on several human cancer cell lines. Fluorescence BBN probes were developed by conjugating the last eight residues of BBN, -Q-W-A-V-G-H-L-M-(NH2), with Alexa Flour 680 or Alexa Fluor 750 dye molecules via amino acid linker -G-G-G. The SD-OCT imaging can identify normal tissue and tumor tissue through the difference in scattering coefficient, and trace the BBN conjugate probes through the absorption of the dye molecules using the twowavelength algorithm. We performed the specific uptake and receptor-blocking experiments of the optical BBN probes in severely compromised immunodeficient mouse model bearing human PC-3 prostate tumor xenografts. Tumor and muscle tissues were collected and used for SD-OCT imaging. The SD-OCT images showed fluorescence traces of the BBN probes in the peptide-targeted tumor tissues. Our results demonstrated that SD-OCT is a potential tool for preclinical and clinical early cancer detection.

Paper Details

Date Published: 22 February 2011
PDF: 8 pages
Proc. SPIE 7890, Advanced Biomedical and Clinical Diagnostic Systems IX, 78900V (22 February 2011); doi: 10.1117/12.874079
Show Author Affiliations
Lixin Ma, Univ. of Missouri-Columbia (United States)
Harry S. Truman Veterans' Memorial Hospital (United States)
Miao Zhang, Univ. of Missouri-Columbia (United States)
Ping Yu, Univ. of Missouri-Columbia (United States)


Published in SPIE Proceedings Vol. 7890:
Advanced Biomedical and Clinical Diagnostic Systems IX
Anita Mahadevan-Jansen; Tuan Vo-Dinh; Warren S. Grundfest, Editor(s)

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