Share Email Print
cover

Proceedings Paper

Fluorescence imaging of macrophages in atherosclerotic plaques using plasmonic gold nanorose
Author(s): Tianyi Wang; Veronika Sapozhnikova; J. Jacob Mancuso; Brian Willsey; Jinze Qiu; Li L. Ma; Xiankai Li; Keith P. Johnston; Marc D. Feldman; Thomas E. Milner
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Macrophages are one of the most important cell types involved in the progression of atherosclerosis which can lead to myocardial infarction. To detect macrophages in atherosclerotic plaques, plasmonic gold nanorose is introduced as a nontoxic contrast agent for fluorescence imaging. We report macrophage cell culture and ex vivo tissue studies to visualize macrophages targeted by nanorose using scanning confocal microscopy. Atherosclerotic lesions were created in the aorta of a New Zealand white rabbit model subjected to a high cholesterol diet and double balloon injury. The rabbit was injected with nanoroses coated with dextran. A HeNe laser at 633 nm was used as an excitation light source and a acousto-optical beam splitter was utilized to collect fluorescence emission in 650-760 nm spectral range. Results of scanning confocal microscopy of macrophage cell culture and ex vivo tissue showed that nanoroses produce a strong fluorescence signal. The presence of nanorose in ex vivo tissue was further confirmed by photothermal wave imaging. These results suggest that scanning confocal microscopy can identify the presence and location of nanorose-loaded macrophages in atherosclerotic plaques.

Paper Details

Date Published: 18 February 2011
PDF: 8 pages
Proc. SPIE 7883, Photonic Therapeutics and Diagnostics VII, 788331 (18 February 2011); doi: 10.1117/12.874076
Show Author Affiliations
Tianyi Wang, The Univ. of Texas at Austin (United States)
Veronika Sapozhnikova, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
J. Jacob Mancuso, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
Brian Willsey, The Univ. of Texas at Austin (United States)
Jinze Qiu, The Univ. of Texas at Austin (United States)
Li L. Ma, The Univ. of Texas at Austin (United States)
Xiankai Li, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
Keith P. Johnston, The Univ. of Texas at Austin (United States)
Marc D. Feldman, The Univ. of Texas Health Science Ctr. at San Antonio (United States)
Thomas E. Milner, The Univ. of Texas at Austin (United States)


Published in SPIE Proceedings Vol. 7883:
Photonic Therapeutics and Diagnostics VII
Kenton W. Gregory; Guillermo J. Tearney; Laura Marcu; Nikiforos Kollias; Bernard Choi; Haishan Zeng; Andreas Mandelis; Henry Hirschberg; Steen J. Madsen; Hyun Wook Kang; Bodo E. Knudsen; Anita Mahadevan-Jansen; E. Duco Jansen; Brian Jet-Fei Wong; Justus F. R. Ilgner, Editor(s)

© SPIE. Terms of Use
Back to Top