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Proceedings Paper

Synthesis of gold nanoparticle based beacon for measurement of STAT5b protein expression
Author(s): Jianpeng Xue; Lingling Shan; Zhenzhen Tu; Hongyan Zhu; Haiyan Chen; Dawei Deng; Zhiyu Qian; Yueqing Gu
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Paper Abstract

Signal transducer and activator of transcription (STAT) 5b is an important protein in JAK-STAT signal pathway and is responsible for the metastasis and proliferation of tumor cells. The determination of the STAT5b expression provides a way to study the mechanism of tumor progress. In this study, gold nanoparticles with different diameters were conjugated to the fluorescein modified STAT5b specific DNA sequence to form the beacon. The procedures for the beacon with better fluorescence properties were optimized. The fluorescence quenching and the recovery properties after hybridizing with mRNA of STAT5b were intensively investigated. Results indicate the gold nanoparticle based beacon is an effective probe for the determination of STAT5b protein expression in JAK-STAT signal pathway and has great potential in the study of drug screening and discovery.

Paper Details

Date Published: 11 February 2011
PDF: 6 pages
Proc. SPIE 7910, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III, 79100I (11 February 2011); doi: 10.1117/12.874014
Show Author Affiliations
Jianpeng Xue, China Pharmaceutical Univ. (China)
Lingling Shan, China Pharmaceutical Univ. (China)
Zhenzhen Tu, China Pharmaceutical Univ. (China)
Hongyan Zhu, China Pharmaceutical Univ. (China)
Haiyan Chen, China Pharmaceutical Univ. (China)
Dawei Deng, China Pharmaceutical Univ. (China)
Zhiyu Qian, Nanjing Univ. of Aeronautics and Astronautics (China)
Yueqing Gu, China Pharmaceutical Univ. (China)

Published in SPIE Proceedings Vol. 7910:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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