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Proceedings Paper

High-speed focal modulation microscopy using acousto-optical modulators for visualization of thick biological specimens
Author(s): Shau Poh Chong; Guangjun Gao; Colin J. R. Sheppard; Nanguang Chen
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Paper Abstract

In recent development of fluorescence microscopy, the out-of-focus fluorescence background that arises when imaging deep inside biological tissues is critical in determining the image quality and penetration depth. Focal modulation microscopy (FMM) is an advanced fluorescence technique that can provide high subcellular resolution when imaging thick specimens mainly by preserving the signal-to-background ratio.

Paper Details

Date Published: 11 February 2011
PDF: 5 pages
Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 79040C (11 February 2011); doi: 10.1117/12.873996
Show Author Affiliations
Shau Poh Chong, National Univ. of Singapore (Singapore)
Guangjun Gao, National Univ. of Singapore (Singapore)
Colin J. R. Sheppard, National Univ. of Singapore (Singapore)
Nanguang Chen, National Univ. of Singapore (Singapore)


Published in SPIE Proceedings Vol. 7904:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)

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