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Proceedings Paper

Target cell specific antibody-based photosensitizers for photodynamic therapy
Author(s): Lauren T. Rosenblum; Makoto Mitsunaga; John W. Kakareka; Nicole Y. Morgan; Thomas J. Pohida; Peter L. Choyke; Hisataka Kobayashi
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Paper Abstract

In photodynamic therapy (PDT), localized monochromatic light is used to activate targeted photosensitizers (PS) to induce cellular damage through the generation of cytotoxic species such as singlet oxygen. While first-generation PS passively targeted malignancies, a variety of targeting mechanisms have since been studied, including specifically activatable agents. Antibody internalization has previously been employed as a fluorescence activation system and could potentially enable similar activation of PS. TAMRA, Rhodamine-B and Rhodamine-6G were conjugated to trastuzumab (brand name Herceptin), a humanized monoclonal antibody with specificity for the human epidermal growth factor receptor 2 (HER2), to create quenched PS (Tra-TAM, Tra-RhoB, and Tra-Rho6G). Specific PDT with Tra-TAM and Tra-Rho6G, which formed covalently bound H-dimers, was demonstrated in HER2+ cells: Minimal cell death (<6%) was observed in all treatments of the HER2- cell line (BALB/3T3) and in treatments the HER2+ cell line (3T3/HER2) with light or trastuzumab only. There was significant light-induced cell death in HER2 expressing cells using Tra-TAM (3% dead without light, 20% at 50 J/cm2, 46% at 100 J/cm2) and Tra-Rho6G (5% dead without light, 22% at 50 J/cm2, 46% at 100 J/cm2). No efficacy was observed in treatment with Tra-RhoB, which was also non-specifically taken up by BALB/3T3 cells and which had weaker PS-antibody interactions (as demonstrated by visualization of protein and fluorescence on SDS-PAGE).

Paper Details

Date Published: 5 February 2011
PDF: 7 pages
Proc. SPIE 7910, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III, 791008 (5 February 2011); doi: 10.1117/12.873887
Show Author Affiliations
Lauren T. Rosenblum, National Institutes of Health (United States)
Makoto Mitsunaga, National Institutes of Health (United States)
John W. Kakareka, National Institutes of Health (United States)
Nicole Y. Morgan, National Institutes of Health (United States)
Thomas J. Pohida, National Institutes of Health (United States)
Peter L. Choyke, National Institutes of Health (United States)
Hisataka Kobayashi, National Institutes of Health (United States)


Published in SPIE Proceedings Vol. 7910:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications III
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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