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Proceedings Paper

Differential CARS microscopy with linearly chirped femtosecond laser pulses
Author(s): Wolfgang Langbein; Israel Rocha-Mendoza; Francesco Masia; Claudia Di Napoli; Iestyn Pope; Peter Watson; Paola Borri
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Paper Abstract

We demonstrate frequency differential CARS (D-CARS) using femtosecond laser pulses linearly chirped by glass elements of high group-velocity dispersion. By replicating the Pump-Stokes pair into a pulse train at twice the laser repetition rate, and controlling the instantaneous frequency difference by glass dispersion, we adjust the Raman frequency probed by each pair in an intrinsically stable way. The resulting CARS intensities are detected simultaneously by a single photomultiplier as sum and difference using lock-in detection. We demonstrate imaging of living cells with strongly suppressed non-resonant background. We also show D-CARS using a single femtosecond laser source.

Paper Details

Date Published: 11 February 2011
PDF: 7 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031I (11 February 2011); doi: 10.1117/12.873872
Show Author Affiliations
Wolfgang Langbein, Cardiff Univ. (United Kingdom)
Israel Rocha-Mendoza, Cardiff Univ. (United Kingdom)
Francesco Masia, Cardiff Univ. (United Kingdom)
Claudia Di Napoli, Cardiff Univ. (United Kingdom)
Iestyn Pope, Cardiff Univ. (United Kingdom)
Peter Watson, Cardiff Univ. (United Kingdom)
Paola Borri, Cardiff Univ. (United Kingdom)


Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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