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Proceedings Paper

Study of photosensitizers pharmacokinetics in mouse tumor model by transillumination fluorescence imaging in vivo
Author(s): Marina V. Shirmanova; Irina V. Balalaeva; Marina A. Sirotkina; Natalya Yu. Lekanova; Ilya V. Turchin; Elena V. Zagainova
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Paper Abstract

In this work we demonstrate an efficiency of transillumination fluorescence imaging for intravital study of photosensitizers pharmacokinetics in tumor-bearing mice. Experiments were performed on CBA mice with subcutaneously transplanted cervical carcinoma. Photosensitizer fotoditazin was used in the therapeutic dose of 10 mg/kg, i.v. Measuring the fluorescence in the whole tumors at different times after injection of photosensitizer, we obtained in vivo data about the drug accumulation and elimination. It was found in vivo that the period of maximum uptake of the photosensitizer in the mouse tumor is from 1 to 8 h post-injection, and its concentration in the tumor tissue at this time is about 2 μg/g. An accumulation of the photosensitizer in the tumor was confirmed by standard methods ex vivo. Noninvasive assessment of pharmacokinetics by transillumination imaging setup provides an opportunity for rapid and cost-effective studies of newly developed photosensitizers.

Paper Details

Date Published: 10 February 2011
PDF: 8 pages
Proc. SPIE 7886, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XX, 78860U (10 February 2011); doi: 10.1117/12.873870
Show Author Affiliations
Marina V. Shirmanova, Nizhny Novgorod State Univ. (Russian Federation)
Nizhny Novgorod State Medical Academy (Russian Federation)
Irina V. Balalaeva, Nizhny Novgorod State Univ. (Russian Federation)
Marina A. Sirotkina, Nizhny Novgorod State Univ. (Russian Federation)
Nizhny Novgorod State Medical Academy (Russian Federation)
Natalya Yu. Lekanova, Nizhny Novgorod State Univ. (Russian Federation)
Ilya V. Turchin, Institute of Applied Physics (Russian Federation)
Elena V. Zagainova, Nizhny Novgorod State Medical Academy (Russian Federation)


Published in SPIE Proceedings Vol. 7886:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XX
David H. Kessel; Tayyaba Hasan, Editor(s)

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