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Proceedings Paper

Adaptive optics for wide-field microscopy
Author(s): C. Bourgenot; C. D. Saunter; J. M. Girkin; G. D. Love
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Paper Abstract

We report on recent developments in the use of adaptive optics (AO) in wide-field microscopy to remove both system and sample induced aberrations. We describe progress on using both a full AO system and image optimization techniques (wavefront sensorless AO). In the latter system the determination of the best mirror shape is found via two routes. In the first an optimization algorithm using a Simplex search pattern is used with an initial random set of mirror shapes. We then explore the use of specific Zernike terms as our starting basis set. In both cases the final optimization performance is not affected by the choice of optimization metric. We then describe an open loop AO system in which the equivalent of a laser guide star is used as the light source for the wavefront sensor.

Paper Details

Date Published: 23 February 2011
PDF: 7 pages
Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 790414 (23 February 2011); doi: 10.1117/12.873857
Show Author Affiliations
C. Bourgenot, Durham Univ. (United Kingdom)
C. D. Saunter, Durham Univ. (United Kingdom)
J. M. Girkin, Durham Univ. (United Kingdom)
G. D. Love, Durham Univ. (United Kingdom)

Published in SPIE Proceedings Vol. 7904:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)

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