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Proceedings Paper

Detection of pathogenic DNA at the single-molecule level
Author(s): Idir Yahiatène; Tobias Klamp; Mark Schüttpelz; Markus Sauer
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Paper Abstract

We demonstrate ultrasensitive detection of pathogenic DNA in a homogeneous assay at the single-molecule level applying two-color coincidence analysis. The target molecule we quantify is a 100 nucleotide long synthetic single-stranded oligonucleotide adapted from Streptococcus pneumoniae, a bacterium causing lower respiratory tract infections. Using spontaneous hybridization of two differently fluorescing Molecular Beacons we demonstrate a detection sensitivity of 100 fM (10-13M) in 30 seconds applying a simple microfluidic device with a 100 μm channel and confocal two-color fluorescence microscopy.

Paper Details

Date Published: 23 February 2011
PDF: 7 pages
Proc. SPIE 7905, Single Molecule Spectroscopy and Imaging IV, 79050M (23 February 2011); doi: 10.1117/12.873850
Show Author Affiliations
Idir Yahiatène, Univ. of Bielefeld (Germany)
Tobias Klamp, Julius-Maximilians-Univ. Würzburg (Germany)
Mark Schüttpelz, Univ. of Bielefeld (Germany)
Markus Sauer, Julius-Maximilians-Univ. Würzburg (Germany)

Published in SPIE Proceedings Vol. 7905:
Single Molecule Spectroscopy and Imaging IV
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann, Editor(s)

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