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Proceedings Paper

Non-radiative excitation fluorescence: driving biology beyond the diffraction limit
Author(s): Pascale Winckler; Rodolphe Jaffiol; Jérôme Plain
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Paper Abstract

We propose a new method of local illumination based on the activation of glass substrates, enables us to drive fluorescence investigations on biological sample at nanometric scale. This local fluorescence excitation is achieved through a non-radiative energy transfer. We demonstrate the potentiality of our technique through two biological relevant applications: imaging of the cell adhesion points and fluorescence correlation spectroscopy in a sub-diffracted volume (attoliter range).

Paper Details

Date Published: 4 February 2011
PDF: 10 pages
Proc. SPIE 7908, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, 790802 (4 February 2011); doi: 10.1117/12.873846
Show Author Affiliations
Pascale Winckler, Lab. de Nanotechnologie et d'Instrumentation Optique, LRC CEA, CNRS, Univ. de Technologie Troyes (France)
Rodolphe Jaffiol, Lab. de Nanotechnologie et d'Instrumentation Optique, LRC CEA, CNRS, Univ. de Technologie Troyes (France)
Jérôme Plain, Lab. de Nanotechnologie et d'Instrumentation Optique, LRC CEA, CNRS, Univ. de Technologie Troyes (France)


Published in SPIE Proceedings Vol. 7908:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII
Alexander N. Cartwright; Dan V. Nicolau, Editor(s)

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