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Proceedings Paper

Live cell single-molecule and superresolution imaging of proteins in bacteria
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Paper Abstract

By providing spatial localization on the nanometer scale, eliminating the need for ensemble averaging, and permitting non-invasive intracellular investigations, single-molecule imaging has brought much insight to biophysics. A particularly enticing application for single-molecule imaging is the capability to investigate live cells and to examine structure and dynamics in the natural environment. To obtain true superresolution, control of the emission of the single molecules provides a way to maintain a sparse concentration of emitters for any frame so that sequential imaging leads to a final reconstruction with information beyond the optical diffraction limit. In this paper, we discuss several single-molecule- based fluorescence methods that are possible, and indeed often enabled, by having live cell specimens.

Paper Details

Date Published: 23 February 2011
PDF: 8 pages
Proc. SPIE 7905, Single Molecule Spectroscopy and Imaging IV, 79050Q (23 February 2011); doi: 10.1117/12.873809
Show Author Affiliations
Julie S. Biteen, Stanford Univ. (United States)
Univ. of Michigan (United States)
W. E. Moerner, Stanford Univ. (United States)

Published in SPIE Proceedings Vol. 7905:
Single Molecule Spectroscopy and Imaging IV
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann, Editor(s)

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