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Proceedings Paper

Validation of artificial skin equivalents as in vitro testing systems
Author(s): Robert Schmitt; Ulrich Marx; Heike Walles; Lena Schober
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Paper Abstract

With the increasing complexity of the chemical composition of pharmaceuticals, cosmetics and everyday substances, the awareness of potential health issues and long term damages for humanoid organs is shifting into focus. Artificial in vitro testing systems play an important role in providing reliable test conditions and replacing precarious animal testing. Especially artificial skin equivalents ASEs are used for a broad spectrum of studies like penetration, irritation and corrosion of substances. One major challenge in tissue engineering is the qualification of each individual ASE as in vitro testing system. Due to biological fluctuations, the stratum corneum hornified layer of some ASEs may not fully develop or other defects might occur. For monitoring these effects we developed an fully automated Optical Coherence Tomography device. Here, we present different methods to characterize and evaluate the quality of the ASEs based on image and data processing of OCT B-scans. By analysing the surface structure, defects, like cuts or tears, are detectable. A further indicator for the quality of the ASE is the morphology of the tissue. This allows to determine if the skin model has reached the final growth state. We found, that OCT is a well suited technology for automatically characterizing artificial skin equivalents and validating the application as testing system.

Paper Details

Date Published: 19 February 2011
PDF: 8 pages
Proc. SPIE 7897, Optical Interactions with Tissue and Cells XXII, 78971B (19 February 2011); doi: 10.1117/12.873804
Show Author Affiliations
Robert Schmitt, RWTH Aachen Univ. (Germany)
Fraunhofer Institute for Production Technology (Germany)
Ulrich Marx, Fraunhofer Institute for Production Technology (Germany)
Heike Walles, Fraunhofer Institute for Interfacial Engineering and Biotechnology (Germany)
Lena Schober, Fraunhofer Institute for Interfacial Engineering and Biotechnology (Germany)


Published in SPIE Proceedings Vol. 7897:
Optical Interactions with Tissue and Cells XXII
E. Duco Jansen; Robert J. Thomas, Editor(s)

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