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Proceedings Paper

Two-photon excitation in life sciences: neurotransmitter and fluorescence uncaging
Author(s): Frédéric Bolze; Jean-François Nicoud; Sylvestre Gug; Sébastien Charon; Alexandre Specht; Maurice Goeldner; David Warther; Xiao-Hua Sun; Pascal Kessler; Yves Lutz; Jean-Luc Vonesch; Attila Losonczy
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Paper Abstract

We describe the molecular engineering of new highly efficient two-photon photolabile cages, based on dissymmetrical donor-acceptor and symmetrical donor-donor systems. Complete characterizations of these new cages, based on a biphenyl, fluorenyl or a 2,5-diphenylthienyl central core will be presented. In vitro photochemistry will be described (uncaging quantum yield, two-photon uncaging action cross-section,...) and we will then focus on biological results obtained in cell culture and on acute brain slices (with glutamate and fluorescence uncaging).

Paper Details

Date Published: 23 February 2011
PDF: 8 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 790339 (23 February 2011); doi: 10.1117/12.873742
Show Author Affiliations
Frédéric Bolze, Lab. de Biphotonique et Pharmacologie, CNRS, Univ. de Strasbourg (France)
Jean-François Nicoud, Lab. de Biphotonique et Pharmacologie, CNRS, Univ. de Strasbourg (France)
Sylvestre Gug, Lab. de Biphotonique et Pharmacologie, CNRS, Univ. de Strasbourg (France)
Lab. de Conception et Application de Molécules Bioactives, CNRS, Univ. de Strasbourg (France)
Sébastien Charon, Lab. de Biphotonique et Pharmacologie, CNRS, Univ. de Strasbourg (France)
Lab. de Conception et Application de Molécules Bioactives, CNRS, Univ. de Strasbourg (France)
Alexandre Specht, Lab. de Conception et Application de Molécules Bioactives, CNRS, Univ. de Strasbourg (France)
Maurice Goeldner, Lab. de Conception et Application de Molécules Bioactives, CNRS, Univ. de Strasbourg (France)
David Warther, Lab. de Conception et Application de Molécules Bioactives, CNRS, Univ. de Strasbourg (France)
Xiao-Hua Sun, Lab. de Biphotonique et Pharmacologie, CNRS, Univ. de Strasbourg (France)
Pascal Kessler, Institut de Genetique et Biologie Moleculaire et Cellulaire (France)
Yves Lutz, Institut de Genetique et Biologie Moleculaire et Cellulaire (France)
Jean-Luc Vonesch, Institut de Genetique et Biologie Moleculaire et Cellulaire (France)
Attila Losonczy, Columbia Univ. (United States)


Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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