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Proceedings Paper

In vivo multiphoton imaging of collagen remodeling after microablative fractional rejuvenation
Author(s): Riccardo Cicchi; Dimitrios Kapsokalyvas; Michela Troiano; Piero Campolmi; Cristiano Morini; Alessandro Cosci; Daniela Massi; Torello Lotti; Francesco S. Pavone
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Paper Abstract

The potential of multiphoton microscopy in providing in-vivo early diagnosis of skin lesions has already been demonstrated, while its capability in therapy follow-up has not been deeply explored so far. Two-photon excited fluorescence and second-harmonic generation microscopy were used in combination to follow-up collagen remodeling after laser micro-ablative rejuvenation. Treated regions of volunteers were imaged with multiphoton microscopy before and after treatment, and we found a strong age-dependence of the treatment effectiveness. In particular, the photorejuvenating effect was negligible in young subjects (< 30 years), whereas a significant production of new collagen was observed in aged subjects (> 70 years). Quantification of the amount of newly produced collagen and its organization were performed by means of visual examination of two-photon images. The obtained results demonstrate the performance of laser fractional micro-ablative rejuvenation without the need of an invasive biopsy as well as the wide applicability range of applications for multiphoton microscopy in clinical dermatology.

Paper Details

Date Published: 17 February 2011
PDF: 8 pages
Proc. SPIE 7883, Photonic Therapeutics and Diagnostics VII, 78830V (17 February 2011); doi: 10.1117/12.873684
Show Author Affiliations
Riccardo Cicchi, Univ. of Florence (Italy)
Dimitrios Kapsokalyvas, Univ. of Florence (Italy)
Michela Troiano, Univ. of Florence (Italy)
Piero Campolmi, Univ. of Florence (Italy)
Cristiano Morini, Univ. of Florence (Italy)
Alessandro Cosci, Univ. of Florence (Italy)
Daniela Massi, Univ. of Florence (Italy)
Torello Lotti, Univ. of Florence (Italy)
Francesco S. Pavone, Univ. of Florence (Italy)

Published in SPIE Proceedings Vol. 7883:
Photonic Therapeutics and Diagnostics VII
Kenton W. Gregory M.D.; Nikiforos Kollias; Andreas Mandelis; Henry Hirschberg M.D.; Hyun Wook Kang; Anita Mahadevan-Jansen; Brian Jet-Fei Wong M.D.; Justus F. R. Ilgner M.D.; Bodo E. Knudsen M.D.; E. Duco Jansen; Steen J. Madsen; Guillermo J. Tearney; Bernard Choi; Haishan Zeng; Laura Marcu, Editor(s)

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