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Proceedings Paper

High contrast coherent anti-Stokes Raman scattering microscopy using tightly focused cylindrical vector beams
Author(s): Jian Lin; Fake Lu; Wei Zheng; Zhiwei Huang
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Paper Abstract

We report on a unique annular aperture detection scheme in radially and linearly polarized coherent anti-Stokes Raman scattering (RP- and LP-CARS) microscopy to effectively remove the solvent background for high contrast vibrational imaging. Our finite-difference time-domain (FDTD) calculations show that the far-field CARS radiation from the scatterer with size comparable to the excitation wavelength is stronger than that from the solvent at large cone angles (from 45° to the maximum collection angle of the objective). The annular detection provides about one to two orders higher contrast for forward detected RP- and LP-CARS microscopy.

Paper Details

Date Published: 22 February 2011
PDF: 4 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031F (22 February 2011); doi: 10.1117/12.873674
Show Author Affiliations
Jian Lin, National Univ. of Singapore (Singapore)
Fake Lu, National Univ. of Singapore (Singapore)
Wei Zheng, National Univ. of Singapore (Singapore)
Zhiwei Huang, National Univ. of Singapore (Singapore)

Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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