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Proceedings Paper

A multispectral FLIM tomograph for in-vivo imaging of skin cancer
Author(s): C. B. Talbot; R. Patalay; I. Munro; H. G. Breunig; K. König; Y. Alexandrov; S. Warren; A. Chu; G. W. Stamp; M. A. A. Neil; Paul M. W. French; Christopher Dunsby
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Paper Abstract

To aid the in vivo diagnosis of skin lesions, we present the design and implementation of a 4 channel FLIM detector and a hyperspectral imaging detector into a clinically licensed commercial two-photon tomograph. We have also implemented image segmentation algorithms to facilitate the automated processing of the large volumes of data produced. The first detector is based on multispectral time correlated single photon counting, providing four channel fluorescence lifetime images. The second detector is a prism-based CCD hyperspectral imager. These detectors provide the capability to extract the relative content and state of autofluorescence compounds present in biological tissue.

Paper Details

Date Published: 28 February 2011
PDF: 9 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79032B (28 February 2011); doi: 10.1117/12.873567
Show Author Affiliations
C. B. Talbot, Imperial College London (United Kingdom)
R. Patalay, Imperial College London (United Kingdom)
I. Munro, Imperial College London (United Kingdom)
H. G. Breunig, JenLab GmbH (Germany)
K. König, JenLab GmbH (Germany)
Y. Alexandrov, Imperial College London (United Kingdom)
S. Warren, Imperial College London (United Kingdom)
A. Chu, Imperial College Healthcare NHS Trust (United Kingdom)
G. W. Stamp, The Royal Marsden Hospital NHS Trust (United Kingdom)
M. A. A. Neil, Imperial College London (United Kingdom)
Paul M. W. French, Imperial College London (United Kingdom)
Christopher Dunsby, Imperial College London (United Kingdom)

Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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