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Proceedings Paper

Efficient delivery of small interfering RNA into injured spinal cords in rats by photomechanical waves
Author(s): Takahiro Ando; Shunichi Sato; Terushige Toyooka; Hiroaki Kobayashi; Hiroshi Nawashiro; Hiroshi Ashida; Minoru Obara
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Paper Abstract

In the central nervous system, lack of axonal regeneration leads to permanent functional disabilities. In spinal cord injury (SCI), the over-expressions of intermediate filament (IF) proteins, such as glial fibrillary acidic protein (GFAP) and vimentin, are mainly involved in glial scar formation; these proteins work as both physical and biochemical barriers to axonal regeneration. Thus, silencing of these IF proteins would be an attractive strategy to treat SCI. In this study, we first attempted to deliver fluorescent probe-labeled siRNAs into injured spinal cords in rats by applying photomechanical waves (PMWs) to examine the capability of PMWs as a tool for siRNA delivery. Intense fluorescence from siRNAs was observed in much broader regions in the spinal cords with PMW application when compared with those with siRNA injection alone. Based on this result, we delivered siRNAs for GFAP and vimentin into injured spinal tissues in rats by applying PMWs. The treatment resulted in efficient silencing of the proteins at five days after SCI and a decrease of the cavity area in the injured tissue at three weeks after SCI when compared with those with siRNA injection alone. These results demonstrate the capability of PMWs for efficient delivery of siRNAs into injured spinal cords and treatment of SCIs.

Paper Details

Date Published: 18 February 2011
PDF: 8 pages
Proc. SPIE 7883, Photonic Therapeutics and Diagnostics VII, 78834S (18 February 2011); doi: 10.1117/12.873513
Show Author Affiliations
Takahiro Ando, Keio Univ. (Japan)
Shunichi Sato, National Defense Medical College (Japan)
Terushige Toyooka, National Defense Medical College (Japan)
Hiroaki Kobayashi, National Defense Medical College (Japan)
Hiroshi Nawashiro, National Defense Medical College (Japan)
Hiroshi Ashida, National Defense Medical College (Japan)
Minoru Obara, Keio Univ. (Japan)

Published in SPIE Proceedings Vol. 7883:
Photonic Therapeutics and Diagnostics VII
Kenton W. Gregory; Nikiforos Kollias; Andreas Mandelis; Henry Hirschberg; Hyun Wook Kang; Anita Mahadevan-Jansen; Brian Jet-Fei Wong; Justus F. R. Ilgner; Bodo E. Knudsen; E. Duco Jansen; Steen J. Madsen; Guillermo J. Tearney; Bernard Choi; Haishan Zeng; Laura Marcu, Editor(s)

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