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Proceedings Paper

Superresolution saturated structured illumination microscopy system: theoretical aspects and real life
Author(s): Dror Fixler; Aviram Gur; Zeev Zalevsky
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Paper Abstract

Many strategies for improving both axial and lateral resolutions are based on a priori information about the input signal and lead on a numerical aperture improvement. But all of them are still limited by the wave nature of light. By using fluorescence technique one theoretically can reach unlimited resolution. In this talk we present simulation and experimental results which show the advantage and problems of using the nonlinear fluorescence effect in super resolution systems. The results show that it is almost impossible to get the nonlinear fluorescence effect without getting being limited by the fluorescence quenching, bleaching and saturation phenomena.

Paper Details

Date Published: 11 February 2011
PDF: 11 pages
Proc. SPIE 7905, Single Molecule Spectroscopy and Imaging IV, 790513 (11 February 2011); doi: 10.1117/12.873245
Show Author Affiliations
Dror Fixler, Bar-Ilan Univ. (Israel)
Aviram Gur, Bar-Ilan Univ. (Israel)
Zeev Zalevsky, Bar-Ilan Univ. (Israel)

Published in SPIE Proceedings Vol. 7905:
Single Molecule Spectroscopy and Imaging IV
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann, Editor(s)

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