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Proceedings Paper

Quadriwave lateral shearing interferometry for quantitative phase microscopy: coupling phase imaging and fluorescence imaging
Author(s): Pierre Bon; Julien Savatier; Benoit Wattellier; Didier Marguet; Serge Monneret
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Paper Abstract

Phase imaging with a high-resolution wavefront sensor is a useful setup for biological imaging. Our setup is based on a quadriwave lateral shearing interferometer mounted on a commercial non-modified transmission white-light microscope. That allows us to make simultaneous measurement in both quantitative transmission phase and fluorescence imaging. We propose here to study co-localization between phase and fluorescence on african green monkey kidney COS7 cells. Phase permits an enhanced visualization of the whole cell and intracellular components while the fluorescence allows a complete identification of each component. Post treatments on phase-shift images are proposed and become very interesting for enhanced visualization of small details such as vesicles or mitochondrias.

Paper Details

Date Published: 22 February 2011
PDF: 6 pages
Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 79040P (22 February 2011); doi: 10.1117/12.873177
Show Author Affiliations
Pierre Bon, Institut Fresnel, CNRS, Aix-Marseille Univ. (France)
PHASICS S.A. (France)
Julien Savatier, Institut Fresnel, CNRS, Aix-Marseille Univ. (France)
Benoit Wattellier, PHASICS S.A. (France)
Didier Marguet, Ctr. Immunologique de Marseille Luminy, CNRS, Univ. de Provence (France)
Serge Monneret, Institut Fresnel, CNRS, Aix-Marseille Univ. (France)


Published in SPIE Proceedings Vol. 7904:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)

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