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Proceedings Paper

Can a one-layer optical skin model including melanin and inhomogeneously distributed blood explain spatially resolved diffuse reflectance spectra?
Author(s): Hanna Karlsson; Anders Pettersson; Marcus Larsson; Tomas Strömberg
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Paper Abstract

Model based analysis of calibrated diffuse reflectance spectroscopy can be used for determining oxygenation and concentration of skin chromophores. This study aimed at assessing the effect of including melanin in addition to hemoglobin (Hb) as chromophores and compensating for inhomogeneously distributed blood (vessel packaging), in a single-layer skin model. Spectra from four humans were collected during different provocations using a twochannel fiber optic probe with source-detector separations 0.4 and 1.2 mm. Absolute calibrated spectra using data from either a single distance or both distances were analyzed using inverse Monte Carlo for light transport and Levenberg-Marquardt for non-linear fitting. The model fitting was excellent using a single distance. However, the estimated model failed to explain spectra from the other distance. The two-distance model did not fit the data well at either distance. Model fitting was significantly improved including melanin and vessel packaging. The most prominent effect when fitting data from the larger separation compared to the smaller separation was a different light scattering decay with wavelength, while the tissue fraction of Hb and saturation were similar. For modeling spectra at both distances, we propose using either a multi-layer skin model or a more advanced model for the scattering phase function.

Paper Details

Date Published: 1 March 2011
PDF: 9 pages
Proc. SPIE 7896, Optical Tomography and Spectroscopy of Tissue IX, 78962Y (1 March 2011); doi: 10.1117/12.873134
Show Author Affiliations
Hanna Karlsson, Linköping Univ. (Sweden)
Anders Pettersson, Perimed AB (Sweden)
Marcus Larsson, Linköping Univ. (Sweden)
Tomas Strömberg, Linköping Univ. (Sweden)


Published in SPIE Proceedings Vol. 7896:
Optical Tomography and Spectroscopy of Tissue IX
Bruce Jason Tromberg; Arjun G. Yodh; Mamoru Tamura; Eva Marie Sevick-Muraca; Robert R. Alfano, Editor(s)

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