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Proceedings Paper

Rotating wall vessel system designed for fluorescent imaging
Author(s): Tristan J. Tayag; S. Dan Dimitrijevich; Lauren C. Del Gallego; Pankaj Kumar
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Paper Abstract

Fluorescent imaging of cells and tissues cultured within a rotating wall vessel bioreactor offers quantitative assessment of the 3-dimensional aggregation of cells into tissue constructs. We present the design of a rotating wall vessel system optimized for real-time fluorescent analysis. The modulation transfer function of our system is found to be superior to the commercially-available vessel used in previous fluorescence imaging studies. We demonstrate dynamic fluorescent imaging of DAPI-stained porcine pancreatic islets.

Paper Details

Date Published: 17 February 2011
PDF: 6 pages
Proc. SPIE 7897, Optical Interactions with Tissue and Cells XXII, 789718 (17 February 2011); doi: 10.1117/12.873091
Show Author Affiliations
Tristan J. Tayag, Texas Christian Univ. (United States)
S. Dan Dimitrijevich, Univ. of North Texas Health Science Ctr. at Fort Worth (United States)
Lauren C. Del Gallego, Texas Christian Univ. (United States)
Pankaj Kumar, Texas Christian Univ. (United States)

Published in SPIE Proceedings Vol. 7897:
Optical Interactions with Tissue and Cells XXII
E. Duco Jansen; Robert J. Thomas, Editor(s)

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