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Proceedings Paper

Subunit rotation in a single FoF1-ATP synthase in a living bacterium monitored by FRET
Author(s): K. Seyfert; T. Oosaka; H. Yaginuma; S. Ernst; H. Noji; R. Iino; M. Börsch
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Paper Abstract

FoF1-ATP synthase is the ubiquitous membrane-bound enzyme in mitochondria, chloroplasts and bacteria which provides the 'chemical energy currency' adenosine triphosphate (ATP) for cellular processes. In Escherichia coli ATP synthesis is driven by a proton motive force (PMF) comprising a proton concentration difference ΔpH plus an electric potential ΔΨ across the lipid membrane. Single-molecule in vitro experiments have confirmed that proton-driven subunit rotation within FoF1-ATP synthase is associated with ATP synthesis. Based on intramolecular distance measurements by single-molecule fluorescence resonance energy transfer (FRET) the kinetics of subunit rotation and the step sizes of the different rotor parts have been unraveled. However, these experiments were accomplished in the presence of a PMF consisting of a maximum ΔpH ~ 4 and an unknown ΔΨ. In contrast, in living bacteria the maximum ΔpH across the plasma membrane is likely 0.75, and ΔΨ has been measured between -80 and -140 mV. Thus the problem of in vivo catalytic turnover rates, or the in vivo rotational speed in single FoF1-ATP synthases, respectively, has to be solved. In addition, the absolute number of functional enzymes in a single bacterium required to maintain the high ATP levels has to be determined. We report our progress of measuring subunit rotation in single FoF1-ATP synthases in vitro and in vivo, which was enabled by a new labeling approach for single-molecule FRET measurements.

Paper Details

Date Published: 1 March 2011
PDF: 9 pages
Proc. SPIE 7905, Single Molecule Spectroscopy and Imaging IV, 79050K (1 March 2011); doi: 10.1117/12.873066
Show Author Affiliations
K. Seyfert, Univ. Stuttgart (Germany)
T. Oosaka, Osaka Univ. (Japan)
H. Yaginuma, Osaka Univ. (Japan)
S. Ernst, Univ. Stuttgart (Germany)
H. Noji, Osaka Univ. (Japan)
Univ. of Tokyo (Japan)
R. Iino, Osaka Univ. (Japan)
M. Börsch, Univ. Stuttgart (Germany)


Published in SPIE Proceedings Vol. 7905:
Single Molecule Spectroscopy and Imaging IV
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann, Editor(s)

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