Share Email Print

Proceedings Paper

Raman spectroscopy: a powerful tool for the non-contact discrimination of bone marrow mesenchymal stem cells and fibroblasts
Author(s): Marieke Pudlas; Steffen Koch; Carsten Bolwien; Thomas Hirth; Heike Walles; Katja Schenke-Layland
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Bone-marrow mesenchymal stem cells (BM-MSCs) are a promising cell source for regenerative medicine. However, it is challenging to determine whether isolated BM-MSC populations are free of fibroblasts. We employed traditional methods and Raman spectroscopy to distinguish BM-MSCs and human dermal fibroblasts (hDFs). Although in vitro differentiation assays revealed the multipotent character of BM-MSCs, long culture periods are a major disadvantage. Using Raman spectroscopy, we could quickly distinguish between BM-MSCs and hDFs. Therefore we conclude that this method is sufficient for the rapid detection of fibroblastic contaminations in BM-MSC cultures.

Paper Details

Date Published: 11 February 2011
PDF: 5 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79032I (11 February 2011); doi: 10.1117/12.873047
Show Author Affiliations
Marieke Pudlas, Fraunhofer IGB (Germany)
Univ. of Stuttgart (Germany)
Steffen Koch, Fraunhofer IGB (Germany)
Univ. of Stuttgart (Germany)
Carsten Bolwien, Fraunhofer IPM (Germany)
Thomas Hirth, Fraunhofer IGB (Germany)
Univ. of Stuttgart (Germany)
Heike Walles, Fraunhofer IGB (Germany)
Julius-Maximillians Univ. Würzburg (Germany)
Katja Schenke-Layland, Fraunhofer IGB (Germany)

Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

© SPIE. Terms of Use
Back to Top