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Proceedings Paper

Water deficit and salt stress diagnosis through LED induced chlorophyll fluorescence analysis in Jatropha curcas L. oil plants for biodiesel
Author(s): Artur S. Gouveia-Neto; Elias A. Silva; Ronaldo A. Oliveira; Patrícia C. Cunha; Ernande B. Costa; Terezinha J. R. Câmara; Lilia G. Willadino
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Paper Abstract

Light-emitting-diode induced chlorophyll fluorescence analysis is employed to investigate the effect of water and salt stress upon the growth process of physicnut(jatropha curcas) grain oil plants for biofuel. Red(Fr) and far-red (FFr) chlorophyll fluorescence emission signals around 685 nm and 735 nm, respectively, were observed and examined as a function of the stress intensity(salt concentration and water deficit) for a period of time of 30 days. The chlorophyll fluorescence(ChlF) ratio Fr/FFr which is a valuable nondestructive and nonintrusive indicator of the chlorophyll content of leaves was exploited to monitor the level of stress experienced by the jatropha plants. The ChlF technique data indicated that salinity plays a minor role in the chlorophyll concentration of leaves tissues for NaCl concentrations in the 25 to 200 mM range, and results agreed quite well with those obtained using conventional destructive spectrophotometric methods. Nevertheless, for higher NaCl concentrations a noticeable decrease in the Chl content was observed. The Chl fluorescence ratio analysis also permitted detection of damage caused by water deficit in the early stages of the plants growing process. A significant variation of the Fr/FFr ratio was observed sample in the first 10 days of the experiment when one compared control and nonwatered samples. The results suggest that the technique may potentially be applied as an early-warning indicator of stress caused by water deficit.

Paper Details

Date Published: 11 February 2011
PDF: 10 pages
Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79020A (11 February 2011); doi: 10.1117/12.872991
Show Author Affiliations
Artur S. Gouveia-Neto, Univ. Federal Rural de Pernambuco (Brazil)
Elias A. Silva, Univ. Federal Rural de Pernambuco (Brazil)
Ronaldo A. Oliveira, Univ. Federal Rural de Pernambuco (Brazil)
Patrícia C. Cunha, Univ. Federal Rural de Pernambuco (Brazil)
Ernande B. Costa, Univ. Federal Rural de Pernambuco (Brazil)
Terezinha J. R. Câmara, Univ. Federal Rural de Pernambuco (Brazil)
Lilia G. Willadino, Univ. Federal Rural de Pernambuco (Brazil)

Published in SPIE Proceedings Vol. 7902:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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