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Proceedings Paper

Diagnosing hepatocellular carcinoma with the intensity and the lifetime of two-photon red autofluorescences
Author(s): Tzu-Ming Liu; Chien-Tai Hsieh; Yu-Shing Chen; Fu-Lien Huang; Hsin-Yi Huang; Wen-Jeng Lee; Chun-Ta Kung; Chi-Kuang Sun
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Paper Abstract

We demonstrated that the intensity levels and lifetimes of two-photon autofluorescences (2PAF) in human liver tissues can be exploited to diagnose hepatocellular carcinoma (HCC). Excited by an infrared femtosecond laser, we suppressed the two-photon autofluorescences of most endogenous fluorophores and made red autofluorescences more specific to particular molecules in the cryo-sectioned human livers. Using such fluorescence contrast, we found HCC tissues have reduced levels of 2PAF and longer fluorescence lifetime.

Paper Details

Date Published: 11 February 2011
PDF: 6 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79032Z (11 February 2011); doi: 10.1117/12.872690
Show Author Affiliations
Tzu-Ming Liu, National Taiwan Univ. (Taiwan)
Chien-Tai Hsieh, National Taiwan Univ. (Taiwan)
Yu-Shing Chen, National Taiwan Univ. (Taiwan)
Fu-Lien Huang, National Taiwan Univ. (Taiwan)
Hsin-Yi Huang, National Taiwan Univ. Hospital (Taiwan)
Wen-Jeng Lee, National Taiwan Univ. Hospital (Taiwan)
Chun-Ta Kung, National Taiwan Univ. (Taiwan)
Chi-Kuang Sun, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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