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Proceedings Paper

Single-photon sensitive fast ebCMOS camera system for multiple-target tracking of single fluorophores: application to nano-biophotonics
Author(s): Thomas Cajgfinger; Eric Chabanat; Agnes Dominjon; Quang T. Doan; Cyrille Guerin; Julien Houles; Remi Barbier
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Paper Abstract

Nano-biophotonics applications will benefit from new fluorescent microscopy methods based essentially on super-resolution techniques (beyond the diffraction limit) on large biological structures (membranes) with fast frame rate (1000 Hz). This trend tends to push the photon detectors to the single-photon counting regime and the camera acquisition system to real time dynamic multiple-target tracing. The LUSIPHER prototype presented in this paper aims to give a different approach than those of Electron Multiplied CCD (EMCCD) technology and try to answer to the stringent demands of the new nano-biophotonics imaging techniques. The electron bombarded CMOS (ebCMOS) device has the potential to respond to this challenge, thanks to the linear gain of the accelerating high voltage of the photo-cathode, to the possible ultra fast frame rate of CMOS sensors and to the single-photon sensitivity. We produced a camera system based on a 640 kPixels ebCMOS with its acquisition system. The proof of concept for single-photon based tracking for multiple single-emitters is the main result of this paper.

Paper Details

Date Published: 16 February 2011
PDF: 15 pages
Proc. SPIE 7875, Sensors, Cameras, and Systems for Industrial, Scientific, and Consumer Applications XII, 78750O (16 February 2011); doi: 10.1117/12.872396
Show Author Affiliations
Thomas Cajgfinger, IPNL, CNRS, Univ. de Lyon (France)
Eric Chabanat, IPNL, CNRS, Univ. de Lyon (France)
Agnes Dominjon, IPNL, CNRS, Univ. de Lyon (France)
Quang T. Doan, IPNL, CNRS, Univ. de Lyon (France)
Cyrille Guerin, IPNL, CNRS, Univ. de Lyon (France)
Julien Houles, IPNL, CNRS, Univ. de Lyon (France)
Remi Barbier, IPNL, CNRS, Univ. de Lyon (France)


Published in SPIE Proceedings Vol. 7875:
Sensors, Cameras, and Systems for Industrial, Scientific, and Consumer Applications XII
Ralf Widenhorn; Valérie Nguyen, Editor(s)

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