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Proceedings Paper

Approach to quantitative detection of CD146 with the label-free protein biosensor based on imaging ellipsometry
Author(s): Yu Niu; Li Liu; Xiyun Yan; Gang Jin
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Paper Abstract

CD146 glycoprotein belonging to cell adhesion molecules is considered to be a novel target on endothelial cell involved in tumor angiogenesis. The biosensor based on imaging ellipsometry (BIE) which is performed in null and off-null mode is used for CD146 detection as a trial by the following steps. Firstly, anti-CD146 antibody as ligand is immobilized on Protein G modified silicon substrate. Then, CD146 test is carried out and its calibration curve is established for the requirement of quantitative detection. Finally, 18 serum samples are detected quantitatively and their results are validated by ELISA's. The sensitivity for CD146 detection achieves the order of ng/ml and the relationship between BIE signal y (grayscale value) and CD146 concentration x (ng/ml) is y=3.3ln(x) +91.3. Compared with ELISA's, the majority of results are in agreement, and the results of two approaches have significant statistic relevance.

Paper Details

Date Published: 16 February 2011
PDF: 7 pages
Proc. SPIE 7875, Sensors, Cameras, and Systems for Industrial, Scientific, and Consumer Applications XII, 78750R (16 February 2011); doi: 10.1117/12.871972
Show Author Affiliations
Yu Niu, Institute of Mechanics (China)
Graduate Univ. of the Chinese Academy of Sciences (China)
Li Liu, Institute of Mechanics (China)
Graduate Univ. of the Chinese Academy of Sciences (China)
Xiyun Yan, Institute of Biophysics (China)
Gang Jin, Institute of Mechanics (China)


Published in SPIE Proceedings Vol. 7875:
Sensors, Cameras, and Systems for Industrial, Scientific, and Consumer Applications XII
Ralf Widenhorn; Valérie Nguyen, Editor(s)

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