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Proceedings Paper

Development of a novel stigmatic imaging mass spectrometer using laser ionization and a multi-turn time-of-flight mass spectrometer
Author(s): Kunio Awazu; Hisanao Hazama; Hidetoshi Yoshimura; Jun Aoki; Hirofumi Nagao; Michisato Toyoda; Katsuyoshi Masuda; Kenichi Fujii; Toshio Tashima; Yasuhide Naito
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Paper Abstract

A stigmatic and microscopic imaging mass spectrometer has been developed using matrix-assisted laser desorption/ionization and a multi-turn time-of-flight mass spectrometer, MULTUM-IMG. Ion images of rhodamine 6G masked by fine grids with pitches of 63.5, 25.4, and 12.7 μm were clearly observed in the linear mode. Separation of stigmatic ion images according to the time-of-flight, i.e., the mass-to-charge ratio of ions was also successfully demonstrated with a micro-dot pattern made with crystal violet and methylene blue. Stigmatic ion images of a micro-dot pattern made with crystal violet was also observed and the ion image of the pattern was maintained after 10 cycles in MULTUM-IMG. A section of a mouse brain stained with crystal violet and methylene blue was observed in the linear mode, and the stigmatic total ion image of crystal violet and methylene blue was in good agreement with the optical microphotograph of the hippocampus in the section.

Paper Details

Date Published: 24 November 2010
PDF: 7 pages
Proc. SPIE 7376, Laser Applications in Life Sciences, 73760Y (24 November 2010); doi: 10.1117/12.871210
Show Author Affiliations
Kunio Awazu, Osaka Univ. (Japan)
JST, CREST (Japan)
Hisanao Hazama, Osaka Univ. (Japan)
JST, CREST (Japan)
Hidetoshi Yoshimura, Osaka Univ. (Japan)
JST, CREST (Japan)
Jun Aoki, Osaka Univ. (Japan)
JST, CREST (Japan)
Hirofumi Nagao, Osaka Univ. (Japan)
JST, CREST (Japan)
Michisato Toyoda, Osaka Univ. (Japan)
JST, CREST (Japan)
Katsuyoshi Masuda, Suntory Institute for Bioorganic Research (Japan)
JST, CREST (Japan)
Kenichi Fujii, Osaka Institute of Technology (Japan)
JST, CREST (Japan)
Toshio Tashima, JST, CREST (Japan)
Yasuhide Naito, The Graduate School for the Creation of New Photonics Industries (Japan)
JST, CREST (Japan)

Published in SPIE Proceedings Vol. 7376:
Laser Applications in Life Sciences
Matti Kinnunen; Risto Myllylä, Editor(s)

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