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Proceedings Paper

The development of SPR device for biomarker detection
Author(s): Pirkko Tervo; Janne Katainen; Adama Sesay; Vesa Virtanen
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Paper Abstract

We have built an imaging SPR device with incident angle calibration system allowing selection of the most sensitive measurement area. Applying the device, an assay for salivary immunoglobulin A (IgA) was developed. Surface plasmon resonance (SPR) provides a highly sensitive, fast and label-free detection method for biomarkers. The specific binding of measured analyte onto the active bio-sensitive layer of the SPR sensor surface causes a change in the refractive index. Imaging SPR allows the analysis of the several different immobilization areas at the same time. Our imaging SPR device utilises a 750nm superluminescent light emitting diode (SLED) with manual incident angle calibration system, which enables us to choose the most sensitive measurement area. This makes the measurement faster and more sensitive for samples of varying refractive index. We demonstrated the applicability of the instrument by developing an immunoassay detection method for immunoglobulin A. IgA is a dominant immunoglobulin in secretions in the mucosal immune system and it has a role in upper respiratory tract infection (URTI). It is also a marker of physical stress.

Paper Details

Date Published: 24 November 2010
PDF: 7 pages
Proc. SPIE 7376, Laser Applications in Life Sciences, 737604 (24 November 2010); doi: 10.1117/12.871118
Show Author Affiliations
Pirkko Tervo, Univ. of Oulu (Finland)
Janne Katainen, Univ. of Oulu (Finland)
Adama Sesay, Univ. of Oulu (Finland)
Vesa Virtanen, Univ. of Oulu (Finland)

Published in SPIE Proceedings Vol. 7376:
Laser Applications in Life Sciences
Matti Kinnunen; Risto Myllylä, Editor(s)

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