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Proceedings Paper

Development of diagnostic SPR based biosensor for the detection of pharmaceutical compounds in saliva
Author(s): Susanna Sonny; Adama M. Sesay; Vesa Virtanen
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Paper Abstract

The aim of the study is to develop diagnostic tests for the detection of pharmaceutical compounds in saliva. Oral fluid is increasingly being considered as an ideal sample matrix. It can be collected non-invasively and causes less stress to the person being tested. The detection of pharmaceutical compounds and drugs in saliva can give valuable information on individual bases on dose response, usage, characterization and clinical diagnostics. Surface plasmon resonance (SPR) is a highly sensitive, fast and label free analytical technique for the detection of molecular interactions. The specific binding of measured analyte onto the active gold sensing surface of the SPR device induces a refractive index change that can be monitored. To monitor these pharmaceutical compounds in saliva the immunoassays were developed using a SPR instrument. The instrument is equipped with a 670nm laser diode and has two sensing channels. Monoclonal antibodies against the pharmaceutical compounds were used to specifically recognise and capture the compounds which intern will have an effect of the refractive index monitored. Preliminary results show that the immunoassays for cocaine and MDMA (3,4-methylenedioxymethamphetamine) are very sensitive and have linear ranges of 0.01 pg/ml - 1 ng/ml and 0.1 pg/ml - 100 ng/ml, respectively.

Paper Details

Date Published: 25 November 2010
PDF: 6 pages
Proc. SPIE 7376, Laser Applications in Life Sciences, 737605 (25 November 2010); doi: 10.1117/12.871116
Show Author Affiliations
Susanna Sonny, Univ. of Oulu (Finland)
Adama M. Sesay, Univ. of Oulu (Finland)
Vesa Virtanen, Univ. of Oulu (Finland)


Published in SPIE Proceedings Vol. 7376:
Laser Applications in Life Sciences

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