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Proceedings Paper

Label-free imaging of human breast tissues using coherent anti-Stokes Raman scattering microscopy
Author(s): Yaliang Yang; Liang Gao; Zhiyong Wang; Michael J Thrall; Pengfei Luo; Kelvin K Wong; Stephen T.C. Wong
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Paper Abstract

Breast cancer is a common disease in women. Current imaging and diagnostic methods for breast cancer confront several limitations, like time-consuming, invasive and with a high cost. Alternative strategies are in high demand to alleviate patients' trauma and lower medical expenses. Coherent anti-Stokes Raman scattering (CARS) imaging technique offers many advantages, including label-free, sub-wavelength spatial resolution and video-rate imaging speed. Therefore, it has been demonstrated as a powerful tool for various biomedical applications. In this study, we present a label-free fast imaging method to identify breast cancer and its subtypes using CARS microscopy. Human breast tissues, including normal, benign and invasive carcinomas, were imaged ex vivo using a custom-built CARS microscope. Compared with results from corresponding hematoxylin and eosin (H&E) stains, the CARS technique has demonstrated its capability in identifying morphological features in a similar way as in H&E stain. These features can be used to distinguish breast cancer from normal and benign tissues, and further separate cancer subtypes from each other. Our pilot study suggests that CARS microscopy could be used as a routine examination tool to characterize breast cancer ex vivo. Moreover, its label-free and fast imaging properties render this technique as a promising approach for in vivo and real-time imaging and diagnosis of breast cancer.

Paper Details

Date Published: 11 February 2011
PDF: 6 pages
Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79032G (11 February 2011); doi: 10.1117/12.871063
Show Author Affiliations
Yaliang Yang, The Methodist Hospital Research Institute (United States)
Liang Gao, The Methodist Hospital Research Institute (United States)
Rice Univ. (United States)
Zhiyong Wang, The Methodist Hospital Research Institute (United States)
Michael J Thrall, The Methodist Hospital (United States)
Pengfei Luo, The Methodist Hospital Research Institute
Kelvin K Wong, The Methodist Hospital Research Institute (United States)
The Methodist Hospital (United States)
Stephen T.C. Wong, The Methodist Hospital Research Institute (United States)
The Methodist Hospital (United States)
Rice Univ. (United States)


Published in SPIE Proceedings Vol. 7903:
Multiphoton Microscopy in the Biomedical Sciences XI
Ammasi Periasamy; Karsten König; Peter T. C. So, Editor(s)

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