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Proceedings Paper

New staining methods for yeast like fungi under special consideration of human pathogenic fungi
Author(s): Astrid Paulitsch-Fuchs; Fritz Treiber; Erik Grasser; Walter Buzina; Christian Rosker
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Paper Abstract

A new method for in-cellular staining of yeast like fungi with Oregon Green and SYTOX Green is presented enabling their detection as well as the observation of cellular details via confocal laser scanning microscopy. Fluorochromes play an important role in many scientific disciplines including medicine, cell biology and botany. For the visualisation of fungal cell walls Calcofluor White is the flourochrome of choice. The necessity of an UV laser for its excitation makes it unpracticable for daily use. Safranin O, DAPI, 2NBDG, Ethidium Bromide and Acridin-orange are commonly used stains for nuclei in fugal microscopy. The attention was given to the possibility of using the differences in staining patterns to distinguish certain pathogenic yeast species e.g. Candida albicans and Candida krusei. Our results show that high quality microscopy of yeast like organisms can readily be achieved by the use of two suitable fluorochromes.

Paper Details

Date Published: 24 November 2010
PDF: 7 pages
Proc. SPIE 7376, Laser Applications in Life Sciences, 73760W (24 November 2010); doi: 10.1117/12.871057
Show Author Affiliations
Astrid Paulitsch-Fuchs, Wetsus, Ctr. of Excellence for Sustainable Water Technology (Netherlands)
Medical Univ. of Graz (Austria)
Fritz Treiber, Medical Univ. of Graz (Austria)
Erik Grasser, Medical Univ. of Graz (Austria)
Walter Buzina, Medical Univ. of Graz (Austria)
Christian Rosker, Univ. of Bern (Switzerland)

Published in SPIE Proceedings Vol. 7376:
Laser Applications in Life Sciences
Matti Kinnunen; Risto Myllylä, Editor(s)

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