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Proceedings Paper

Anti-HIV-1 activities of photodynamic therapy using hematoporphyrin monomethyl ether
Author(s): Huijuan Yin; Yingxin Li; Yongtang Zheng; Xuying Ye; Chunyan Li; Zhaohui Zou; Yu Zhao
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Paper Abstract

Photodynamic therapy (PDT) with a new photosensitizer, hematoporphyrin monomethyl ether (HMME) was investigated as a potential inactivator of human immunodeficiency virus (HIV) in vitro. A series of concentrations of HMME were mixed with MT4, C8166 or H9/HIV-1IIIB cells or HIV-1IIIB cell-free virus for 2 h. Cells or virus were irradiated with a 630 nm semiconductor laser. The antiviral effects were assayed by counting the syncytial formation or detecting the viability of cells by MTT assay or measuring the p24 antigen expression level in supernatant by ELISA. All effects observed with HMME-PDT were compared with treatments with methylene blue (MB-PDT). PDT inhibited the cell-cell membrane fusion up to 64.68% for HMME-PDT and 61.56% for MB-PDT. Likewise, virus-cell fusion was inhibited by up to 85% for HMME-PDT and 73.64% for MB-PDT. Cell-free virus inhibition of up to 100% was achieved by each treatment. However, PDT had little effect on the acute infection or chronic infection of HIV-1IIIB. Therefore, PDT can effectively inhibit cell-free HIV particle infectivity and can also inhibit membrane fusion induced by HIV-1IIIB. These results suggest that PDT may be a promising new treatment for HIV infected individuals, especially those with high viral load.

Paper Details

Date Published:
PDF: 10 pages
Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 78451E; doi: 10.1117/12.870695
Show Author Affiliations
Huijuan Yin, Institute of Biomedical Engineering (China)
Yingxin Li, Institute of Biomedical Engineering (China)
Yongtang Zheng, Kunming Institute of Zoology (China)
Xuying Ye, Tianjin First Ctr. Hospital (China)
Chunyan Li, Kunming Institute of Zoology (China)
Zhaohui Zou, Institute of Biomedical Engineering (China)
Yu Zhao, Institute of Biomedical Engineering (China)

Published in SPIE Proceedings Vol. 7845:
Optics in Health Care and Biomedical Optics IV
Qingming Luo; Ying Gu; Xingde Li, Editor(s)

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