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Proceedings Paper

Fluoroimmunoassay for antigen based on fluorescence quenching between quantum dots and gold nanoparticles
Author(s): Peng Huang; Kan Wang; Omar Pandoli; Xueqing Zhang; Feng Gao; Jun Shao; Xiaogang You; Rong He; Hua Song; Daxiang Cui
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Paper Abstract

A unique, sensitive, and highly specific fluoroimmunoassay system for antigen detection using gold and quantum dot nanoparticles has been developed. The assay is based on the fluorescence quenching of quantum dots caused by gold nanoparticles coated with antibody. To demonstrate its analytical capabilities, the CdTe quantum dots were coated with anti-HBsAg monoclonal antibodies (QDs-MAb1) and gold nanoparticles coated with another anti-HBsAg monoclonal antibodies (GNPs-MAb2) which specifically bound with HBsAg could sandwich the HBsAg captured by the immunoreactions. The sandwich-type immunocomplex was formed and the fluorescence intensity of quantum dots was measured. The results showed that the fluorescence intensity of quantum dots at 570 nm was negative linear proportional to the HBsAg concentration logarithm, and the limit of detection of the HBsAg was 0.928 ng/mL. This new system can be extended to detect target molecules with matched antibodies and has broad potential applications in immunoassay and disease diagnosis.

Paper Details

Date Published: 8 November 2010
PDF: 5 pages
Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 784514 (8 November 2010); doi: 10.1117/12.870360
Show Author Affiliations
Peng Huang, Shanghai Jiao Tong Univ. (China)
Kan Wang, Shanghai Jiao Tong Univ. (China)
Omar Pandoli, Shanghai Jiao Tong Univ. (China)
Xueqing Zhang, Shanghai Jiao Tong Univ. (China)
Feng Gao, Shanghai Jiao Tong Univ. (China)
Jun Shao, Shanghai Jiao Tong Univ. (China)
Xiaogang You, Shanghai Jiao Tong Univ. (China)
Rong He, Shanghai Jiao Tong Univ. (China)
Hua Song, Shanghai Jiao Tong Univ. (China)
Daxiang Cui, Shanghai Jiao Tong Univ. (China)

Published in SPIE Proceedings Vol. 7845:
Optics in Health Care and Biomedical Optics IV
Qingming Luo; Ying Gu; Xingde Li, Editor(s)

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