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Proceedings Paper

Autofluorescence characteristics of human leukemia cells and mononuclear cells
Author(s): Lifu Xiao; Xiaoying Liao; Lisheng Lin; Huifang Huang; Yuanzhong Chen; Buhong Li
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Paper Abstract

Autofluorescence spectroscopy is a powerful method to identify the endogenous fluorophores in normal and cancerous cells. The purpose of this study is to characterize the autofluorescence spectra of human normal and leukemia cells. Autofluorescence measurements of each cell line are performed over a wide range of cell concentrations. All of the leukemia cells indicate a statistically significant increase in the tryptophan fluorescence relative to that of the normal cells, while no statistically significant differences are observed in the reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD) fluorescence between the normal and leukemia cells. The results suggest that the differences in autofluorescence spectra for leukemia cells and mononuclear cells may be attributed in part to differences in endogenous fluorophores of different cells.

Paper Details

Date Published: 8 November 2010
PDF: 6 pages
Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 78452G (8 November 2010); doi: 10.1117/12.870282
Show Author Affiliations
Lifu Xiao, Fujian Normal Univ. (China)
Xiaoying Liao, Fujian Medical Univ. (China)
Lisheng Lin, Fujian Normal Univ. (China)
Huifang Huang, Fujian Medical Univ. (China)
Yuanzhong Chen, Fujian Medical Univ. (China)
Buhong Li, Fujian Normal Univ. (China)


Published in SPIE Proceedings Vol. 7845:
Optics in Health Care and Biomedical Optics IV
Qingming Luo; Ying Gu; Xingde Li, Editor(s)

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