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Proceedings Paper

Kinetic analysis of singlet oxygen generation in a living cell using Singlet Oxygen Sensor Green
Author(s): Yi Shen; Huiyun Lin; Zufang Huang; Lifu Xiao; Defu Chen; Buhong Li; Shusen Xie
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Paper Abstract

Singlet oxygen (1O2) can be generated in a living cell upon focused laser irradiation of an intracellular photosensitizer. In this study, 1O2 generation from the plasma membrane-targeted protoporphyrin IX (PpIX) in human nasopharyngeal carcinoma CNE2 cells was monitored indirectly by using the fluorescence probe Singlet Oxygen Sensor Green agent (SOSG). The confocal images indicate that the fluorescence of SOSG in the vicinity of the cells that incubated with PpIX was dramatically enhanced with the increased irradiation time, while there is no significant enhancement for the control cells. Moreover, the fluorescence of SOSG is dramatically enhanced with the increase of the intracellular PpIX in CNE2 cells for the same photoirradiation time. These observations imply that the 1O2 generated from the plasma membrane-targeted PpIX in the CNE2 cells can be escaped into the extracellular medium and to react with the SOSG to produce SOSG-EP, and the fluorescence enhancement of SOSG around the cells mainly depends on the intracellular PpIX. Our findings may be useful for further monitored the 1O2 that can be escaping from the living cells.

Paper Details

Date Published: 9 November 2010
PDF: 6 pages
Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 78451F (9 November 2010); doi: 10.1117/12.870261
Show Author Affiliations
Yi Shen, Fujian Normal Univ. (China)
Huiyun Lin, Fujian Normal Univ. (China)
Zufang Huang, Fujian Normal Univ. (China)
Lifu Xiao, Fujian Normal Univ. (China)
Defu Chen, Fujian Normal Univ. (China)
Buhong Li, Fujian Normal Univ. (China)
Shusen Xie, Fujian Normal Univ. (China)


Published in SPIE Proceedings Vol. 7845:
Optics in Health Care and Biomedical Optics IV
Qingming Luo; Ying Gu; Xingde Li, Editor(s)

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