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Proceedings Paper

Surfaced-enhanced Raman spectroscopy of nasopharyngeal carcinoma cell using gol nanoparticles
Author(s): Rong Chen; Hao Huang; Li-qing Sun; Jian-ji Pan; Wei-wei Chen; Ying Su; Shang-yuan Feng; Yong-zeng Li
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Paper Abstract

The surface-enhanced Raman scattering (SERS) spectroscopy and normal Raman spectroscopy of single living human nasopharyngeal carcinoma cells(CNE-1) were tested and analyzed by gold nanoparticles incubation into cells. The characteristic Raman bands in the SERS spectra of living cells were tentatively assigned. Six obvious Raman bands (718, 1001, 1123, 1336, 1446, 1660 cm-1) were observed in the normal Raman spectroscopy of living CNE-1 cells. Colloidal gold nanoparticles that were introduced inside cells resulted in strongly enhanced Raman signals of the native chemical constituents of the cells, and over twenty SERS Raman bands were observed in the SERS spectroscopy of living CNE-1 cells. Four vibrations of the DNA backbone (1026, 1097, 1336 and 1585 cm-1) confirmed that some gold nanoparticles were able to enter the nucleus. The results showed that, based on colloidal gold, the SERS spectroscopy might provide a sensitive and structurally selective detecting method for native chemicals inside a cell, such as DNA and phenylalanine.

Paper Details

Date Published: 8 November 2010
PDF: 6 pages
Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 784524 (8 November 2010); doi: 10.1117/12.869049
Show Author Affiliations
Rong Chen, Fujian Normal Univ. (China)
Hao Huang, Fujian Univ. of Traditional Chinese Medicine (China)
Li-qing Sun, Fuzhou First Hospital (China)
Jian-ji Pan, Fujian Provincial Tumor Hospital (China)
Wei-wei Chen, Fujian Normal Univ. (China)
Fujian Univ. of Traditional Chinese Medicine (China)
Ying Su, Fujian Provincial Tumor Hospital (China)
Shang-yuan Feng, Fujian Normal Univ. (China)
Yong-zeng Li, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 7845:
Optics in Health Care and Biomedical Optics IV
Qingming Luo; Ying Gu; Xingde Li, Editor(s)

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