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Proceedings Paper

Spintronic microfluidic platform for biomedical and environmental applications
Author(s): F. A. Cardoso; V. C. Martins; L. P. Fonseca; J. Germano; L. A. Sousa; M. S. Piedade; P. P. Freitas
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Paper Abstract

Faster, more sensitive and easy to operate biosensing devices still are a need at important areas such as biomedical diagnostics, food control and environmental monitoring. Recently, spintronic-devices have emerged as a promising alternative to the existent technologies [1-3]. A number of advantages, namely high sensitivity, easy integration, miniaturization, scalability, robustness and low cost make these devices potentially capable of responding to the existent technological need. In parallel, the field of microfluidics has shown great advances [4]. Microfluidic systems allow the analysis of small sample volumes (from micro- down to pico-liters), often by automate sample processing with the ability to integrate several steps into a single device (analyte amplification, concentration, separation and/or labeling), all in a reduced assay time (minutes to hours) and affordable cost. The merging of these two technologies, magnetoresistive biochips and microfluidics, will enable the development of highly competitive devices. This work reports the integration of a magnetoresistive biochip with a microfluidic system inside a portable and autonomous electronic platform aiming for a fully integrated device. A microfluidic structure fabricated in polydimethylsiloxane with dimensions of W: 0.5mm, H: 0.1mm, L: 10mm, associated to a mechanical system to align and seal the channel by pressure is presented (Fig. 1) [5]. The goal is to perform sample loading and transportation over the chip and simultaneously control the stringency and uniformity of the wash-out process. The biochip output is acquired by an electronic microsystem incorporating the circuitry to control, address and read-out the 30 spin-valve sensors sequentially (Fig. 1) [2]. This platform is being applied to the detection of water-borne microbial pathogens (e.g. Salmonella and Escherichia coli) and genetic diseases diagnosis (e.g. cystic fibrosis) through DNA hybridization assays. Open chamber measurements were performed as described elsewhere [2]. Briefly, a 20 μl sample droplet is manually dispensed over the chip, limited by a polymeric frame. When using the microfluidic system for sample loading, a known volume of sample is introduced into the fluidic system through the help of a syringe pump at a controlled velocity.

Paper Details

Date Published: 8 September 2010
PDF: 3 pages
Proc. SPIE 7653, Fourth European Workshop on Optical Fibre Sensors, 765306 (8 September 2010); doi: 10.1117/12.868407
Show Author Affiliations
F. A. Cardoso, INESC MN and IN (Portugal)
Instituto Superior Técnico (Portugal)
V. C. Martins, INESC MN and IN (Portugal)
Institute of Biotechnology and Bioengineering (Portugal)
L. P. Fonseca, Institute of Biotechnology and Bioengineering (Portugal)
J. Germano, Instituto Superior Técnico (Portugal)
INESC ID (Portugal)
L. A. Sousa, Instituto Superior Técnico (Portugal)
INESC ID (Portugal)
M. S. Piedade, Instituto Superior Técnico (Portugal)
INESC ID (Portugal)
P. P. Freitas, INESC MN and IN (Portugal)
Instituto Superior Técnico (Portugal)

Published in SPIE Proceedings Vol. 7653:
Fourth European Workshop on Optical Fibre Sensors
José Luís Santos; Brian Culshaw; José Miguel López-Higuera; William N. MacPherson, Editor(s)

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