Share Email Print
cover

Proceedings Paper

Measuring binding kinetics of biomolecular interactions using a localized surface plasmon couple fluorescence fiber optic biosensor
Author(s): Ying-Feng Chang; Jo-Ping Hsieh; Li-Chen Su; Ying-Chang Li; Cheng-Chung Lee; Chien Chou
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

In this study, we describe a novel method for analyzing protein-protein binding kinetics at ultra-low concentration (1 pg/mL) using a localized surface plasmon coupled fluorescence fiber-optic biosensor (LSPCF-FOB). The association and dissociation rate constants, ka and kd, respectively, for the binding kinetics of the mouse IgG/ anti-mouse IgG interaction have been calculated to be ka = (5.9928±3.1540)x106 M-1s-1 and kd = (1.0587±0.5572)x10-3 s-1. The theoretical basis of this analytical approach is a rapid-mixing model integrated with a two-compartment model; has been experimentally verified in this study as well. The LSPCF-FOB provides a potentially alternative option for characterizing the interaction of biomolecules at ultra-low concentrations.

Paper Details

Date Published: 24 August 2010
PDF: 6 pages
Proc. SPIE 7759, Biosensing III, 77590H (24 August 2010); doi: 10.1117/12.863621
Show Author Affiliations
Ying-Feng Chang, National Yang-Ming Univ. (Taiwan)
Chang Gung Univ. (Taiwan)
Jo-Ping Hsieh, National Yang-Ming Univ. (Taiwan)
Chang Gung Univ. (Taiwan)
Li-Chen Su, National Central Univ. (Taiwan)
Chang Gung Univ. (Taiwan)
Ying-Chang Li, National Central Univ. (Taiwan)
Chang Gung Univ. (Taiwan)
Cheng-Chung Lee, National Central Univ. (Taiwan)
Chien Chou, National Yang-Ming Univ. (Taiwan)
Chang Gung Univ. (Taiwan)
National Central Univ. (Taiwan)


Published in SPIE Proceedings Vol. 7759:
Biosensing III
Hooman Mohseni; Manijeh Razeghi, Editor(s)

© SPIE. Terms of Use
Back to Top