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Proceedings Paper

Force measurements with a translating holographic optical trap
Author(s): Louis T McLane; Keith M. Carroll; Jan Scrimgeour; Mauricio D. Bedoya; Anthony Kramer; Jennifer E. Curtis
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Paper Abstract

Force measurements made with a translating holographic optical trap (HOT) of a viscous and a viscoelastic medium are investigated. In purely viscous media, Stokes drag cannot be measured with a translating HOT with established methods. In the viscoelastic system of the pericellular coat, the standard force curves generated by a fixed optical trap coupled with a moving stage can reliably be reproduced by translating HOT experiments. The viscoelastic cell coat provides an example where slow relaxation dynamics makes force measurements relatively insensitive to differences between measurements. These preliminary studies suggest that when the relaxation time scale of a system is much slower than the time scale of the HOT updates, translating HOTs can be reliably used to make force measurements on a viscoelastic, non-equilibrium system.

Paper Details

Date Published: 27 August 2010
PDF: 10 pages
Proc. SPIE 7762, Optical Trapping and Optical Micromanipulation VII, 77621J (27 August 2010); doi: 10.1117/12.863549
Show Author Affiliations
Louis T McLane, Georgia Institute of Technology (United States)
Keith M. Carroll, Georgia Institute of Technology (United States)
Jan Scrimgeour, Georgia Institute of Technology (United States)
Mauricio D. Bedoya, Georgia Institute of Technology (United States)
Anthony Kramer, Georgia Institute of Technology (United States)
Jennifer E. Curtis, Georgia Institute of Technology (United States)


Published in SPIE Proceedings Vol. 7762:
Optical Trapping and Optical Micromanipulation VII
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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