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Proceedings Paper

Rapid detection of cancer related DNA nanoparticulate biomarkers and nanoparticles in whole blood
Author(s): Michael J. Heller; Raj Krishnan; Avery Sonnenberg
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Paper Abstract

The ability to rapidly detect cell free circulating (cfc) DNA, cfc-RNA, exosomes and other nanoparticulate disease biomarkers as well as drug delivery nanoparticles directly in blood is a major challenge for nanomedicine. We now show that microarray and new high voltage dielectrophoretic (DEP) devices can be used to rapidly isolate and detect cfc-DNA nanoparticulates and nanoparticles directly from whole blood and other high conductance samples (plasma, serum, urine, etc.). At DEP frequencies of 5kHz-10kHz both fluorescent-stained high molecular weight (hmw) DNA, cfc-DNA and fluorescent nanoparticles separate from the blood and become highly concentrated at specific DEP highfield regions over the microelectrodes, while blood cells move to the DEP low field-regions. The blood cells can then be removed by a simple fluidic wash while the DNA and nanoparticles remain highly concentrated. The hmw-DNA could be detected at a level of <260ng/ml and the nanoparticles at <9.5 x 109 particles/ml, detection levels that are well within the range for viable clinical diagnostics and drug nanoparticle monitoring. Disease specific cfc-DNA materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia (CLL) and confirmed by PCR genotyping analysis.

Paper Details

Date Published: 24 August 2010
PDF: 4 pages
Proc. SPIE 7759, Biosensing III, 77590P (24 August 2010); doi: 10.1117/12.861579
Show Author Affiliations
Michael J. Heller, Univ. of California, San Diego (United States)
Raj Krishnan, Univ. of California, San Diego (United States)
Avery Sonnenberg, Univ. of California, San Diego (United States)

Published in SPIE Proceedings Vol. 7759:
Biosensing III
Hooman Mohseni; Manijeh Razeghi, Editor(s)

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