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Proceedings Paper

Adsorption of cytochrome c to silica surfaces studied using evanescent wave broadband cavity-enhanced absorption spectroscopy
Author(s): L. J. Moore; L. van der Sneppen; R. Peverall; G. Hancock; G. A. D. Ritchie
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Paper Abstract

The adsorption of cytochrome c (cyt c) to a silica surface has been studied by use of evanescent wave broadband cavityenhanced absorption spectroscopy (EW-BBCEAS). Visible radiation from a supercontinuum source is coupled into an optical cavity consisting of a pair of broadband high reflectivity mirrors, and a total internal reflection (TIR) event at the prism/water interface. Aqueous solutions of cyt c are placed onto the TIR footprint on the prism surface and the subsequent protein adsorption is probed by the resulting evanescent wave. The time integrated cavity output is directed into a spectrometer, where it is dispersed and analysed. The high spectral brilliance of the SC affords a baseline noise comparable to evanescent wave cavity ring-down spectroscopy (EW-CRDS), and the broadband nature of the source allows observation of a wide spectral range (ca 250 nm in the visible). The system is calibrated by measuring the absorption spectra of dyes of a known absorbance. Absorption spectra of cyt c are obtained for both S and P polarized radiation, allowing information about the orientation of the adsorbed protein to be extracted.

Paper Details

Date Published: 30 August 2010
PDF: 8 pages
Proc. SPIE 7758, Physical Chemistry of Interfaces and Nanomaterials IX, 77580G (30 August 2010); doi: 10.1117/12.860715
Show Author Affiliations
L. J. Moore, Univ. of Oxford (United Kingdom)
L. van der Sneppen, Univ. College Cork (Ireland)
R. Peverall, Univ. of Oxford (United Kingdom)
G. Hancock, Univ. of Oxford (United Kingdom)
G. A. D. Ritchie, Univ. of Oxford (United Kingdom)

Published in SPIE Proceedings Vol. 7758:
Physical Chemistry of Interfaces and Nanomaterials IX
Oleg V. Prezhdo, Editor(s)

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