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Proceedings Paper

A simple enzyme based biosensor on flexible plastic substrate
Author(s): Senaka K. Kanakamedala; Haidar T. Alshakhouri; Mangilal Agarwal; Ji Fang; Mark A. DeCoster
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Paper Abstract

An enzyme based biosensor was fabricated by employing a simple, inexpensive and rapid xurography fabrication process. The electrodes and channel were made from the conducting polymer poly(3,4-ethyelenedioxythiphene) poly(styrene sulfonate) (PEDOT:PSS). PEDOT:PSS was selectively deposited using a polyimide tape mask. The tape mask was peeled off from the substrate after annealing the polymer in vacuum. Polymer wells of defined dimensions were made and were attached to the device to accommodate the solutions. This sensor utilizes the change in current as a parameter to measure different analyte concentrations. Initial experiments were done by using the sensor for glucose detection. The sensor is able to detect the glucose concentrations approximately from 1 μM to 10 mM range covering glucose in human saliva (8-210 μM). The glucose oxidase activity was independently measured using colorimetric method and the results indicate that the sensor retains the enzyme activity and can be used as a biosensor to detect various analytes. The analyte of interest can be measured by preloading the corresponding enzyme into the wells.

Paper Details

Date Published: 24 August 2010
PDF: 8 pages
Proc. SPIE 7759, Biosensing III, 77590F (24 August 2010); doi: 10.1117/12.860587
Show Author Affiliations
Senaka K. Kanakamedala, Louisiana Tech Univ. (United States)
Haidar T. Alshakhouri, Louisiana Tech Univ. (United States)
Mangilal Agarwal, Indiana Univ.-Purdue Univ. Indianapolis (United States)
Ji Fang, Louisiana Tech Univ. (United States)
Mark A. DeCoster, Louisiana Tech Univ. (United States)

Published in SPIE Proceedings Vol. 7759:
Biosensing III
Hooman Mohseni; Manijeh Razeghi, Editor(s)

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