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Proceedings Paper

Development of microfluidic system and optical tweezers for electrophysiological investigations of an individual cell
Author(s): A. Alrifaiy; N. Bitaraf; O. Lindahl; K. Ramser
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Paper Abstract

We present a new approach of combining Lab-on-a-chip technologies with optical manipulation technique for accurate investigations in the field of cell biology. A general concept was to develop and combine different methods to perform advanced electrophysiological investigations of an individual living cell under optimal control of the surrounding environment. The conventional patch clamp technique was customized by modifying the open system with a gas-tight multifunctional microfluidics system and optical trapping technique (optical tweezers). The system offers possibilities to measure the electrical signaling and activity of the neuron under optimum conditions of hypoxia and anoxia while the oxygenation state is controlled optically by means of a spectroscopic technique. A cellbased microfluidics system with an integrated patch clamp pipette was developed successfully. Selectively, an individual neuron is manipulated within the microchannels of the microfluidic system under a sufficient control of the environment. Experiments were performed to manipulate single yeast cell and red blood cell (RBC) optically through the microfluidics system toward an integrated patch clamp pipette. An absorption spectrum of a single RCB was recorded which showed that laser light did not impinge on the spectroscopic spectrum of light. This is promising for further development of a complete lab-on-a-chip system for patch clamp measurements.

Paper Details

Date Published: 27 August 2010
PDF: 7 pages
Proc. SPIE 7762, Optical Trapping and Optical Micromanipulation VII, 77622K (27 August 2010); doi: 10.1117/12.860478
Show Author Affiliations
A. Alrifaiy, Luleå Univ. of Technology (Sweden)
N. Bitaraf, Luleå Univ. of Technology (Sweden)
O. Lindahl, Luleå Univ. of Technology (Sweden)
K. Ramser, Luleå Univ. of Technology (Sweden)

Published in SPIE Proceedings Vol. 7762:
Optical Trapping and Optical Micromanipulation VII
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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